Ginsberg H, Le N A, Mays C, Gibson J, Brown W V
Arteriosclerosis. 1981 Nov-Dec;1(6):463-70. doi: 10.1161/01.atv.1.6.463.
We have studied the effect of increased dietary cholesterol on production and degradation of plasma very low density lipoproteins and low density lipoproteins. We studied five unselected normal volunteers who were on weight-maintaining diets consisting of 45% carbohydrate, 40% fat, and 15% protein with a ratio of polyunsaturated to saturated fat of 0.4. Cholesterol content was 150 mg/1000 kcal in Period A and 500 mg/1000 kcal in Period B. Subjects were fed each diet for 4 to 5 weeks. The turnover of apoproteins B in very low density and low density lipoproteins and of triglyceride in very low density lipoproteins were measured during the last 2 weeks of each study period using 131I-labeled very low density lipoproteins and 125I-labeled low density lipoproteins and 2-3H-glycerol, respectively. There was no significant change in plasma total cholesterol or triglycerides or in low density lipoprotein cholesterol levels during the high cholesterol versus the low cholesterol diet. Similarly, high cholesterol intake had no effect on the rate of production or clearance of apo B in very low density and low density lipoproteins or of triglyceride in very low density lipoproteins. These data indicate that the flux of very low density lipoproteins does not change in nonresponders to high cholesterol diets.
我们研究了饮食中胆固醇增加对血浆极低密度脂蛋白和低密度脂蛋白产生及降解的影响。我们对五名未经挑选的正常志愿者进行了研究，他们维持体重的饮食由45%的碳水化合物、40%的脂肪和15%的蛋白质组成，多不饱和脂肪与饱和脂肪的比例为0.4。在A期胆固醇含量为150毫克/1000千卡，在B期为500毫克/1000千卡。每位受试者每种饮食喂养4至5周。在每个研究期的最后两周，分别使用131I标记的极低密度脂蛋白、125I标记的低密度脂蛋白和2-3H-甘油测量极低密度脂蛋白和低密度脂蛋白中载脂蛋白B的周转率以及极低密度脂蛋白中甘油三酯的周转率。在高胆固醇饮食与低胆固醇饮食期间，血浆总胆固醇、甘油三酯或低密度脂蛋白胆固醇水平均无显著变化。同样，高胆固醇摄入对极低密度脂蛋白和低密度脂蛋白中载脂蛋白B的产生或清除率以及极低密度脂蛋白中甘油三酯的产生或清除率均无影响。这些数据表明，在对高胆固醇饮食无反应者中，极低密度脂蛋白的通量没有变化。
