Fontaine A R, Hall B D
J Biomed Mater Res. 1981 Jan;15(1):61-71. doi: 10.1002/jbm.820150110.
The physical and chemical properties of echinoderm skeleton are reviewed. A method is described for preparing cell-free, sterile echinoderm skeletal plates (ossicles) which were used as porous substrates for cell cultures. Ossicles of the starfish Pisaster ochraceus were evaluated as substrates for the culture of three mammalian cell lines. Each line grew vigorously on ossicles, and fibroblasts quickly infiltrated their porous microstructure. Echinoderm skeletal plates provide a simple, convenient alternative to coverslips and porous membranes for SEM or correlated SEM/TEM studies of cell behavior. More importantly, the preliminary evidence for biocompatibility presented suggests that native echinoderm skeleton has potential use as a biomaterial and, because of its microstructure and relative solubility; deserves evaluation as a kind of biodegradable ceramic.
本文综述了棘皮动物骨骼的物理和化学性质。描述了一种制备无细胞、无菌棘皮动物骨骼板（小骨）的方法，这些骨骼板被用作细胞培养的多孔基质。对海星赭色海星的小骨作为三种哺乳动物细胞系培养的基质进行了评估。每个细胞系在小骨上都生长旺盛，成纤维细胞迅速渗透到其多孔微结构中。棘皮动物骨骼板为扫描电子显微镜（SEM）或相关的SEM/透射电子显微镜（TEM）细胞行为研究提供了一种简单、方便的替代盖玻片和多孔膜的材料。更重要的是，所呈现的生物相容性初步证据表明，天然棘皮动物骨骼有作为生物材料的潜在用途，并且由于其微观结构和相对溶解性，值得作为一种可生物降解陶瓷进行评估。
