Hill R J
Biochim Biophys Acta. 1980;595(1):140-5. doi: 10.1016/0005-2736(80)90256-4.
A mutant of Tetrahymena thermophila has been isolated which requires an unsaturated fatty acid for growth. Pellicles isolated from cultures supplemented with palmitoleic, elaidic, oleic, linoleic and gamma-linolenic acids show widely differing membrane fluidities, as measured by the polarisation of fluorescence technique. In contrast to the behaviour of the wild type organism, the changes in fluidity of the membrane, once induced by supplementation, are permanent. This mutant should prove extremely useful for studying structure-function relationships in the various membrane systems of Tetrahymena.
已分离出一种嗜热四膜虫突变体，其生长需要不饱和脂肪酸。从添加了棕榈油酸、反油酸、油酸、亚油酸和γ-亚麻酸的培养物中分离出的表膜，通过荧光偏振技术测量，显示出差异很大的膜流动性。与野生型生物体的行为相反，一旦通过添加诱导，膜流动性的变化是永久性的。这种突变体对于研究嗜热四膜虫各种膜系统中的结构-功能关系应该非常有用。
