Raychowdhury M K, Goswami R, Chakrabarti P
J Appl Bacteriol. 1985 Aug;59(2):183-8. doi: 10.1111/j.1365-2672.1985.tb03319.x.
The growth of two penicillin-resistant Gram-positive bacteria, Bacillus licheniformis (749/C, penicillin G-resistant) and Staphylococcus aureus (metR 18, methicillin-resistant) and one Gram-negative strain, Escherichia coli (cloxacillin-resistant) as well as that of their wild counterparts was inhibited by the long-chain unsaturated fatty acids, linoleic, linolenic and arachidonic acid. The minimum inhibitory concentrations (MIC) of all the fatty acids were found to be 4-6 micrograms/ml for Staph. aureus (metR 18 & wild), 8-30 micrograms/ml for B. licheniformis (749/C & wild) and 70-90 micrograms/ml for E. coli (cloxacillin-resistant & wild). The inhibitory activity increased as the number of double bonds in the fatty acids increased. In most instances the concentrations of fatty acids required to inhibit the growth of the penicillin-resistant strains were lower than that required for their sensitive counterparts. This inhibition of growth in the presence of fatty acids may be due to an increase in permeability of the membrane as evidenced by the measurement of the leakage of 260 nm absorbing material and fluidity.
两种耐青霉素革兰氏阳性菌，地衣芽孢杆菌（749/C，耐青霉素G）和金黄色葡萄球菌（metR 18，耐甲氧西林）以及一种革兰氏阴性菌，大肠杆菌（耐氯唑西林）及其野生型菌株的生长受到长链不饱和脂肪酸，亚油酸、亚麻酸和花生四烯酸的抑制。发现所有脂肪酸对金黄色葡萄球菌（metR 18及野生型）的最低抑菌浓度（MIC）为4-6微克/毫升，对地衣芽孢杆菌（749/C及野生型）为8-30微克/毫升，对大肠杆菌（耐氯唑西林及野生型）为70-90微克/毫升。随着脂肪酸中双键数量的增加，抑制活性增强。在大多数情况下，抑制耐青霉素菌株生长所需的脂肪酸浓度低于其敏感型菌株所需的浓度。在脂肪酸存在下生长受到抑制可能是由于膜通透性增加，这可通过测量260nm吸收物质的泄漏和流动性得到证明。
