Kozin F, McCarty D J
J Lab Clin Med. 1980 Jan;95(1):49-58.
The adsorption of IgG to microcrystalline MSU was studied to determine its potential biologic importance in gouty inflammation. IgG showed high-affinity binding isotherms, suggesting monomolecular adsorption at concentrations of IgG found in inflammatory synovial effusions and possible surface aggregation at higher concentrations. Crystals previously exposed to a variety of other proteins showed no reduction in IgG binding or a modest one. And co-incubation of IgG with other proteins reduced IgG adsorption to only a modest extent. Highly anionic substances such as hyaluronate or PVPNO enhanced IgG binding to crystals. Studies of the functional orientation of adsorbed IgG, by using molecular probes, indicated that the Fc portion of the molecule was fully exposed, suggesting that the sites of attachment to crystal surface residue on the Fab moiety of IgG.
研究了IgG对微晶型尿酸钠（MSU）的吸附作用，以确定其在痛风性炎症中潜在的生物学重要性。IgG呈现出高亲和力结合等温线，表明在炎症性滑膜积液中发现的IgG浓度下为单分子吸附，而在更高浓度下可能发生表面聚集。先前暴露于多种其他蛋白质的晶体，其IgG结合能力未降低或仅有适度降低。并且IgG与其他蛋白质共同孵育仅在适度程度上降低了IgG的吸附。高度阴离子性物质如透明质酸盐或聚乙烯吡咯烷酮N-氧化物（PVPNO）增强了IgG与晶体的结合。通过使用分子探针研究吸附的IgG的功能取向，表明该分子的Fc部分完全暴露，这表明IgG与晶体表面结合的位点位于其Fab部分的残基上。
