Harley C B, Goldstein S
Science. 1980 Jan 11;207(4427):191-3. doi: 10.1126/science.7350654.
The commitment theory of human fibroblast aging predicts that 55 percent of cells will be nondividing at the middle to late stages of the replicative lifespan; in the present study, however, fewer than 10 percent were nondividing. The fact that no immortal diploid cells have yet been reported is also at odds with the theory. Available data on the variable life-span of clones and mass cultures, the dependence of longevity on population size, and the predominance of certain cell types at termination of a culture are compatible with simpler theories, which support the idea that the limited replicative life-span of diploid fibroblasts is a valid model for organismic aging.
人类成纤维细胞衰老的承诺理论预测，在复制寿命的中期到后期，55%的细胞将不再分裂；然而，在本研究中，不分裂的细胞不到10%。尚未有永生二倍体细胞的报道这一事实也与该理论相悖。关于克隆和大规模培养物可变寿命、寿命对群体大小的依赖性以及培养结束时某些细胞类型占优势的现有数据，与更简单的理论相符，这些理论支持二倍体成纤维细胞有限的复制寿命是机体衰老有效模型的观点。
