Webster D F, Harvey W, Dyson M, Pond J B
Ultrasonics. 1980 Jan;18(1):33-7. doi: 10.1016/0041-624x(80)90050-5.
Collagen synthesis by human embryonic fibroblasts in vitro was estimated using a collagenase-sensitivity assay. Collagen synthesis was stimulated by irradiation with ultrasound at a frequency of 3 MHz, a space-time peak intensity of 0.5 Wcm-2, pulsed at a mark-space ratio of 2:8 ms for 5 min at ambient pressure. This stimulation was suppressed by the application of a positive pressure of 2 atmospheres during irradiation of the cells. Increasing the pressure in the absence of ultrasound had no effect on the rate of collagen synthesis in control cells. This stimulation, therefore, appears to be due to ultrasound-induced cavitation, since it is unlikely that increasing the pressure could modify any other ultrasonic parameter. Collagen synthesis is apparently stimulated to the same extent as general protein synthesis.
使用胶原酶敏感性测定法评估人胚胎成纤维细胞在体外的胶原蛋白合成。在环境压力下，以3MHz的频率、0.5W/cm²的时空峰值强度、2:8ms的占空比脉冲超声辐照5分钟可刺激胶原蛋白合成。在细胞辐照期间施加2个大气压的正压可抑制这种刺激。在无超声的情况下增加压力对对照细胞中的胶原蛋白合成速率没有影响。因此，这种刺激似乎是由于超声诱导的空化作用，因为增加压力不太可能改变任何其他超声参数。胶原蛋白合成显然受到与一般蛋白质合成相同程度的刺激。
