人血浆中皮质醇的测定：七种检测方法的评估与比较

The determination of cortisol in human plasma: evaluation and comparison of seven assays.

作者信息

Thijssen J H, van den Berg J H, Adlercreutz H, Gijzen A H, de Jong F H, Meijer J C, Moolenaar A J

出版信息

Clin Chim Acta. 1980 Jan 1;100(1):39-46. doi: 10.1016/0009-8981(80)90183-7.

Abstract

In a comparative study, seven different methods for the determination of cortisol in human plasma were evaluated, using routine patient samples. Four of these methods used radioactive steroids (125I- or 3H-labelled) and in three no radioactivity was needed. For the statistical evaluation a direct 3H-radioimmunoassay was arbitrarily taken as the independent variable. It was found that all other methods correlated well with this assay. However, the simplest method, the fluorimetric, cannot be recommended mainly because of its non-specific fluorescence and troublesome interference from some widely-used drugs. Of the methods evaluated a radioimmunoassay is recommended. For laboratories having no equipment for measurements of radioactivity, the more elaborate and time-consuming fluorometric method of Clark may represent a good alternative.

摘要

在一项比较研究中，使用常规患者样本对七种不同的人血浆皮质醇测定方法进行了评估。其中四种方法使用放射性类固醇（125I或3H标记），另外三种方法则无需放射性。为了进行统计评估，任意选取直接3H放射免疫测定法作为自变量。结果发现，所有其他方法与该测定法相关性良好。然而，最简单的方法，即荧光法，主要因其非特异性荧光以及一些常用药物产生的麻烦干扰而不被推荐。在所评估的方法中，推荐放射免疫测定法。对于没有放射性测量设备的实验室，克拉克更复杂且耗时的荧光法可能是一个不错的选择。