一种用于血浆皮质醇的简单放射免疫测定法。

A simple radioimmunoassay for plasma cortisol.

作者信息

Seth J, Brown L M

出版信息

Clin Chim Acta. 1978 May 16;86(1):109-20. doi: 10.1016/0009-8981(78)90465-5.

Abstract

A simple radioimmunoassay (RIA) for plasma cortisol is described which combines the advantages of (i) direct analysis of untreated plasma samples, (ii) use of solid-coupled anti-cortisol antibodies and (iii) use of a gamma-labelled radioligand. The reagents are relatively easily prepared and stable, and the analysis can be completed in 4 h. Inter-assay precision (C.V.) is 8-11%. Critical examination of specificity using high pressure liquid chromatography showed that 23-35% of the immunoassayable material in plasma was not cortisol. RIA results on samples collected under basal conditions were on average 40 nmol/l lower than fluorimetric results, while in insulin hypoglycaemia and synacthen (ACTH) stimulation tests, this difference increased to over 100 nmol/l. The RIA is technically more simple than fluorimetric, competitive-protein-binding, and many RIA methods, and can be used with advantage in the routine investigation of adrenocortical function. However, using the present antiserum, the RIA is not applicable to investigations on patients receiving metyrapone, nor in suspected cases of congenital adrenal hyperplasia.

摘要

本文描述了一种用于血浆皮质醇的简单放射免疫分析（RIA）方法，该方法具有以下优点：（i）直接分析未经处理的血浆样本；（ii）使用固相偶联的抗皮质醇抗体；（iii）使用γ标记的放射性配体。试剂相对易于制备且稳定，分析可在4小时内完成。批间精密度（变异系数）为8 - 11%。使用高压液相色谱对特异性进行的严格检查表明，血浆中可通过免疫分析检测到的物质有23 - 35%并非皮质醇。基础条件下采集的样本的放射免疫分析结果平均比荧光分析结果低40 nmol/l，而在胰岛素低血糖和促肾上腺皮质激素（ACTH）刺激试验中，这种差异增加到超过100 nmol/l。该放射免疫分析在技术上比荧光分析、竞争性蛋白结合分析以及许多放射免疫分析方法更简单，可有效地用于肾上腺皮质功能的常规检查。然而，使用目前的抗血清，该放射免疫分析不适用于对接受甲吡酮治疗的患者的检测，也不适用于先天性肾上腺皮质增生疑似病例的检测。