Ladefoged K
Clin Chim Acta. 1980 Jan 15;100(2):149-53. doi: 10.1016/0009-8981(80)90076-5.
A simple acid extraction procedure for sample preparation of diet and faeces for zinc analysis by flame atomic absorption spectrophotometry is evaluated. About 0.5 g of homogenized diet or faeces was mixed with 6 ml 1 mol/l hydrochloric acid. After 24 h, 4 ml chloroform and 2 ml methanol was added to dissolve fatty components and reduce surface tension. The samples were centrifuged and the supernatants analysed. The sensitivity of the method was 1.7 mumol per 1000 g. The coefficient of intra-assay variation was for faeces 1.7% (n = 20), for diet 2.1% (n = 25). The coefficient of inter-assay variation was for faeces 4.7% (n = 24), for diet 4.9% (n = 20). Recovery of known amounts of zinc added to faeces was 99.7 +/- 3.1% (n = 50). Recovery of zinc added to diet was 98.9 +/- 2.9% (n = 47). A good agreement was found between zinc determinations by the acid extraction method and by a method of acid digestion.
对一种用于火焰原子吸收分光光度法分析饮食和粪便中锌的样品制备的简单酸提取程序进行了评估。将约0.5 g匀浆后的饮食或粪便与6 ml 1 mol/l盐酸混合。24小时后，加入4 ml氯仿和2 ml甲醇以溶解脂肪成分并降低表面张力。将样品离心并分析上清液。该方法的灵敏度为每1000 g 1.7 μmol。批内变异系数粪便为1.7%（n = 20），饮食为2.1%（n = 25）。批间变异系数粪便为4.7%（n = 24），饮食为4.9%（n = 20）。添加到粪便中的已知量锌的回收率为99.7±3.1%（n = 50）。添加到饮食中的锌的回收率为98.9±2.9%（n = 47）。酸提取法与酸消化法测定锌之间发现有良好的一致性。
