干扰素和（2'-5'）寡腺苷酸合成酶对黑色素瘤细胞生长的调节
Growth regulation of melanoma cells by interferon and (2'-5')oligoadenylate synthetase.
作者信息
Creasey A A, Eppstein D A, Marsh Y V, Khan Z, Merigan T C
出版信息
Mol Cell Biol. 1983 May;3(5):780-6. doi: 10.1128/mcb.3.5.780-786.1983.
Abstract
We report that endogenous, as well as exogenous, interferon (IFN) regulates the growth of human melanoma cells in culture. When antibodies directed against human fibroblast IFN were incorporated into the media of high-density cells stimulated to proliferate with serum, the cells entered the cell cycle earlier than did the controls. In investigating the biochemical basis for this finding, we have found that there is an inverse relationship between the (2'-5')oligoadenylate synthetase levels and the percentage of cells in S in untreated cultures. Upon IFN treatment, the relationship is obliterated and (2'-5')oligoadenylate synthetase levels increase throughout all phases of the cell cycle. This increase in enzyme levels correlates well with the decreased probability of the IFN-treated cells to cycle. These findings suggest a biological role for IFN as a negative growth factor for cells in culture.
摘要
我们报告称，内源性以及外源性干扰素（IFN）可调节培养的人黑色素瘤细胞的生长。当将针对人成纤维细胞IFN的抗体加入到用血清刺激增殖的高密度细胞培养基中时，这些细胞比对照细胞更早进入细胞周期。在研究这一发现的生化基础时，我们发现在未处理的培养物中，（2'-5'）寡腺苷酸合成酶水平与处于S期的细胞百分比之间存在反比关系。经IFN处理后，这种关系消失，并且在细胞周期的所有阶段中（2'-5'）寡腺苷酸合成酶水平均升高。酶水平的这种升高与经IFN处理的细胞进入周期的可能性降低密切相关。这些发现表明IFN作为培养细胞的负生长因子具有生物学作用。
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