Appelkvist E L, Dallner G
Biochim Biophys Acta. 1980 Jan 18;617(1):156-60. doi: 10.1016/0005-2760(80)90233-7.
The localization of beta-oxidation of fatty acids in isolated peroxisomes from rat liver was investigated. The enzyme system is soluble in the luminal compartment and carnitine does not appear to be involved in the transfer of the CoA derivatives through the peroxisomal membrane. Experiments involving proteolysis, inhibitors and competitive inhibition suggest that a fatty acid binding protein is responsible for the carrier process. This carrier protein seems to be present in increased amounts both in the supernatant and in the peroxisomes after clofibrate induction.
对大鼠肝脏分离出的过氧化物酶体中脂肪酸β-氧化的定位进行了研究。该酶系统可溶于腔室，肉碱似乎不参与辅酶A衍生物通过过氧化物酶体膜的转运。涉及蛋白水解、抑制剂和竞争性抑制的实验表明，一种脂肪酸结合蛋白负责载体过程。在氯贝丁酯诱导后，这种载体蛋白似乎在上清液和过氧化物酶体中的含量均增加。
