Coy D H, Gardner J
Int J Pept Protein Res. 1980 Jan;15(1):73-8. doi: 10.1111/j.1399-3011.1980.tb02552.x.
The 28-residue sequence of porcine vasoactive peptide (VIP) was assembled on a benzhydrylamine resin support, cleaved by HF treatment, and purified by ion-exchange and partition chromatography. In addition to the normal criteria, the homogeneity of the final material, obtained in 16% yield, was assessed by reverse-phase high performance liquid chromatography and the isolation and examination of cyanogen bromide cleavage fragments by the same technique. The purified VIP possessed characteristic inhibitory effects on pentagastrin-induced gastric acid release in the dog. Upon storage, even as the lyophilized powder in vacuo, HPLC revealed the slow formation of a contaminant possibly representing deamidated peptide.
猪血管活性肽（VIP）的28个氨基酸残基序列在二苯甲基胺树脂载体上组装，经氢氟酸处理裂解，再通过离子交换和分配色谱法纯化。除了常规标准外，以16%的产率获得的最终产物的纯度，通过反相高效液相色谱法评估，同时通过相同技术对溴化氰裂解片段进行分离和检测。纯化后的VIP对狗体内五肽胃泌素诱导的胃酸分泌具有典型的抑制作用。储存时，即使是真空冻干粉末，高效液相色谱法也显示缓慢形成了一种可能代表脱酰胺肽的污染物。
