Meyers C A, Coy D H
Int J Pept Protein Res. 1980 Oct;16(4):248-53. doi: 10.1111/j.1399-3011.1980.tb02584.x.
The 36-residue sequence of human pancreatic polypeptide (HPP) was synthesized by solid-phase methodology, cleaved from the benzhydrylamine resin by HF treatment, and purified by gel filtration, ion exchange, and partition chromatography. In addition to the usual criteria, the homogeneity of the final product was examined by reversed phase high performance liquid chromatography. This technique was also used to isolate and characterize the cyanogen bromide cleavage fragments, and finally to compare the synthetic product with native human, bovine, and porcine pancreatic polypeptide isolated from natural sources. The purified HPP, like native PP, was a potent inhibitor of pancreatic secretion in the dog.
人胰多肽（HPP）的36个氨基酸残基序列通过固相方法合成，经氢氟酸处理从二苯甲基胺树脂上切割下来，然后通过凝胶过滤、离子交换和分配色谱法进行纯化。除了常规标准外，最终产物的纯度还通过反相高效液相色谱法进行检测。该技术还用于分离和鉴定溴化氰裂解片段，最后将合成产物与从天然来源分离的天然人、牛和猪胰多肽进行比较。纯化后的HPP与天然PP一样，是犬胰液分泌的有效抑制剂。
