Carraway R, Kitabgi P, Leeman S E
J Biol Chem. 1978 Nov 25;253(22):7996-8.
The amino acid sequence of radioimmunoassayable neurotensin, isolated from bovine small intestinal extracts, has been shown to be the same as that of the peptide originally isolated from bovine hypothalamic extracts. This was accomplished by sequence studies on the intact peptide as well as on its chymotryptic and papain-generated fragments. Thus, neurotensin joins the group of biologically active peptides shown to be present in the same molecular form in both brain and intestine.
从牛小肠提取物中分离出的可进行放射免疫测定的神经降压素的氨基酸序列,已被证明与最初从牛下丘脑提取物中分离出的肽的氨基酸序列相同。这是通过对完整肽及其经胰凝乳蛋白酶和木瓜蛋白酶产生的片段进行序列研究来完成的。因此,神经降压素加入了已被证明在脑和肠中以相同分子形式存在的生物活性肽组。