Takaki T
J Cancer Res Clin Oncol. 1980 Jan;96(1):27-33. doi: 10.1007/BF00412894.
A brain metastasis of bronchial squamous cell carcinoma in a patient was cultivated. The cells with epithelium-like characteristics were selected from the primary culture, and were subcultured to establish a cell line. The cultured cells were identified to be neoplastic by means of heterologous transplantation to nude mice, in which tumors developed and reproduced histologic characteristics as were seen in the primary tumor of the patient.
对一名支气管鳞状细胞癌脑转移患者的肿瘤进行培养。从原代培养物中挑选出具有上皮样特征的细胞，并进行传代培养以建立细胞系。通过将培养的细胞异种移植到裸鼠体内来鉴定其为肿瘤性细胞，移植后裸鼠体内长出肿瘤，且肿瘤再现了患者原发肿瘤的组织学特征。
