Kahn S N, Shortman R C, Khan R A, Thompson E J
J Clin Chem Clin Biochem. 1980 Jan;18(1):23-6. doi: 10.1515/cclm.1980.18.1.23.
The method of preparing CSF and dilute serum samples for polyacrylamide gel electrophoresis has a marked effect on the pattern of the resolved proteins. The incorporation of sucrose or glycerol into the sample seriously impairs the quality of the resolution and affects the actual number of bands resolved and the relative mobility of certain proteins. The use of sucrose as an anti-convection medium in dense samples such as serum and CSF is not recommended.
用于聚丙烯酰胺凝胶电泳的脑脊液和稀释血清样本的制备方法对分离出的蛋白质图谱有显著影响。在样本中加入蔗糖或甘油会严重损害分离质量，并影响实际分离出的条带数量以及某些蛋白质的相对迁移率。不建议在血清和脑脊液等浓稠样本中使用蔗糖作为抗对流介质。
