Kubiak E J, Munson J W
J Pharm Sci. 1980 Feb;69(2):152-6. doi: 10.1002/jps.2600690209.
The chromatographic behavior of morphine, codeine, and ethylmorphine was examined using reversed-phase high-performance liquid chromatography and ion-pair formation. Alkyl sulfonates and sulfates significantly increased the retention times for these compounds. The carbon chain length of the pairing ions was linearly related to the log of the capacity factors of these amine drugs. A mechanism for the increased retention based on ion-pair formation in the mobile phase is proposed. The use of dioctyl sodium sulfosuccinate as a pairing ion for codeine is described, and a method utilizing this pairing ion was developed for the quantitation of codeine in syrups. This method was applied successfully to various syrups containing codeine or codeine phosphate.
采用反相高效液相色谱法和离子对形成技术研究了吗啡、可待因和乙基吗啡的色谱行为。烷基磺酸盐和硫酸盐显著增加了这些化合物的保留时间。配对离子的碳链长度与这些胺类药物容量因子的对数呈线性关系。提出了一种基于流动相中离子对形成导致保留增加的机制。描述了使用磺基琥珀酸二辛酯钠作为可待因的配对离子，并开发了一种利用该配对离子定量糖浆中可待因的方法。该方法已成功应用于各种含可待因或磷酸可待因的糖浆。
