血浆中吲哚美辛的葡萄糖氧化酶微量测定法

GLC microdetermination of indomethacin in plasma.

作者信息

Evans M A

出版信息

J Pharm Sci. 1980 Feb;69(2):219-20. doi: 10.1002/jps.2600690231.

Abstract

A GLC method utilizing an electron-capture detector is described for analysis of indomethacin in blood plasma. Indomethacin is extracted with ethyl acetate from plasma buffered to pH 5.0. The ethyl acetate is evaporated to dryness, and indomethacin is derivatized to a pentafluoropropionyl ester prior to chromatography. A GLC standard is used for peak height quantitation of indomethacin. The extraction efficiency from plasma is 92 +/- 3%, and as little as 1 ng of indomethacin can be quantitatively determined.

摘要

描述了一种利用电子捕获检测器的气相色谱法，用于分析血浆中的吲哚美辛。吲哚美辛用乙酸乙酯从缓冲至pH 5.0的血浆中萃取。乙酸乙酯蒸发至干，吲哚美辛在色谱分析前衍生为五氟丙酰酯。使用气相色谱标准品对吲哚美辛进行峰高定量。血浆的萃取效率为92±3%，低至1 ng的吲哚美辛也可进行定量测定。