Brown-Woodman P D, Marley P B, Morris S, Rodger J C, White I G
Arch Androl. 1980 Mar;4(2):149-55. doi: 10.3109/01485018008986482.
The origin of glycerylphosphorylcholine (GPC), N-acetylaminosugar, inositol, and prostaglandins in human seminal plasma was investigated by correlating the concentration of these components in split ejaculates with known marker constituents. Fructose and acid phosphatase were selected as markers of the secretory activity of the seminal vesicles and prostate gland, respectively, and spermatozoa indicated epididymal origin. The concentration of fructose was lowest in the first fraction of the semen and increased to a maximum in the final portion. Prostaglandins E and F and N-acetylaminosugar values closely followed this pattern, indicating that these components originate in the seminal vesicles. The concentration of spermatozoa was high in the first two fractions, decreasing to a minimum in the final fraction. The distribution of GPC was similar to that of the spermatozoa, indicating that the epididymis secretes this compound. Inositol levels were similar in all fractions, indicating that it is probably present in epididymal, vesicular, and prostatic fluid. Human spermatozoa were unable to utilize N-acetylglucosamine or inositol. High concentrations of some prostaglandins (100 micrograms/ml of PGF1 alpha, 15S 15 met. F2 alpha, PGA1, and PGA2) depressed the endogenous oxygen uptake of human spermatozoa.
通过将分段射精中这些成分的浓度与已知标记成分相关联，研究了人精浆中甘油磷酸胆碱（GPC）、N - 乙酰氨基糖、肌醇和前列腺素的来源。分别选择果糖和酸性磷酸酶作为精囊和前列腺分泌活性的标记物，精子表明其来源于附睾。精液第一部分中果糖浓度最低，在最后一部分增加到最大值。前列腺素E和F以及N - 乙酰氨基糖的值紧密遵循这种模式，表明这些成分起源于精囊。精子浓度在前两部分中较高，在最后一部分降至最低。GPC的分布与精子相似，表明附睾分泌这种化合物。肌醇水平在所有部分中相似，表明它可能存在于附睾、精囊和前列腺液中。人类精子无法利用N - 乙酰葡糖胺或肌醇。高浓度的一些前列腺素（100微克/毫升的PGF1α、15S 15 - 甲基 - F2α、PGA1和PGA2）会抑制人类精子的内源性氧摄取。
