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相似文献

1
Ribosome binding sites visualized on freeze-fractured membranes of the rough endoplasmic reticulum.核糖体结合位点在糙面内质网冷冻断裂膜上的可视化。
J Cell Biol. 1980 Apr;85(1):147-52. doi: 10.1083/jcb.85.1.147.
2
Mobility of ribosomes bound to microsomal membranes. A freeze-etch and thin-section electron microscope study of the structure and fluidity of the rough endoplasmic reticulum.结合于微粒体膜的核糖体的移动性。粗面内质网结构与流动性的冷冻蚀刻及超薄切片电子显微镜研究。
J Cell Biol. 1977 Mar;72(3):530-51. doi: 10.1083/jcb.72.3.530.
3
Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. I. Functional tests on rat liver microsomal subfractions.多肽转运装置定位于内质网中含有核糖体结合蛋白和核糖体的区域。I. 对大鼠肝脏微粒体亚组分的功能测试。
J Cell Biol. 1984 Dec;99(6):2247-53. doi: 10.1083/jcb.99.6.2247.
4
Characterization of the ribosomal binding site in rat liver rough microsomes: ribophorins I and II, two integral membrane proteins related to ribosome binding.大鼠肝脏粗面微粒体中核糖体结合位点的特征：核糖体结合糖蛋白I和II，两种与核糖体结合相关的整合膜蛋白。
J Supramol Struct. 1978;8(3):279-302. doi: 10.1002/jss.400080307.
5
A polysome-membrane binding system from rat liver. I. Basic characterization of the binding system.大鼠肝脏的多核糖体-膜结合系统。I. 结合系统的基本特征
J Biochem. 1975 Dec;78(6):1225-33. doi: 10.1093/oxfordjournals.jbchem.a131020.
6
Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. II. Rat liver microsomal subfractions contain equimolar amounts of ribophorins and ribosomes.多肽转运装置定位于内质网中含有核糖体结合蛋白和核糖体的区域。II. 大鼠肝脏微粒体亚组分含有等摩尔量的核糖体结合蛋白和核糖体。
J Cell Biol. 1984 Dec;99(6):2254-9. doi: 10.1083/jcb.99.6.2254.
7
Circular polysomes predominate on the rough endoplasmic reticulum of somatotropes and mammotropes in the rat anterior pituitary.在大鼠垂体前叶的生长激素细胞和催乳激素细胞的糙面内质网上，环状多核糖体占主导地位。
Am J Anat. 1987 Jan;178(1):1-10. doi: 10.1002/aja.1001780102.
8
Compartmentation of the rough endoplasmic reticulum.粗面内质网的区室化
Mol Cell Biochem. 1986 Jun;71(1):3-18. doi: 10.1007/BF00219323.
9
The in vitro reassembly of rough endoplasmic reticulum: ribosome binding capacity.糙面内质网的体外重装配：核糖体结合能力
Mol Biol Rep. 1978 Jun 16;4(2):111-5. doi: 10.1007/BF00775971.
10
Structure of rough, smooth, stripped and reconstituted rough membranes derived from rat liver as visualized by the freeze fracture technique.通过冷冻蚀刻技术观察到的源自大鼠肝脏的粗糙、光滑、剥离和重构粗糙膜的结构。
Mol Biol Rep. 1975 Dec;2(4):311-9. doi: 10.1007/BF00357018.

引用本文的文献

1
Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells.高倍放大下的病毒感染：用于分析受感染细胞结构的三维电子显微镜方法
Viruses. 2015 Dec 3;7(12):6316-45. doi: 10.3390/v7122940.
2
Reconstitution into liposomes of membrane proteins involved in ribosome binding on rough endoplasmic reticulum. Ribosome-binding capacity.参与核糖体与糙面内质网结合的膜蛋白重构入脂质体。核糖体结合能力。
Biochem J. 1981 Mar 15;194(3):907-13. doi: 10.1042/bj1940907.
3
Studies on polysomes synthesizing influenza virus haemagglutinin.关于合成流感病毒血凝素的多核糖体的研究。
Arch Virol. 1982;74(4):299-310. doi: 10.1007/BF01314163.
4
The ultrastructure of the developing inner and outer segments of the photoreceptors of chick embryo retina as revealed by the rapid-freezing and deep-etching techniques.通过快速冷冻和深度蚀刻技术揭示的鸡胚视网膜光感受器发育中的内节和外节的超微结构。
Anat Embryol (Berl). 1984;169(2):141-50. doi: 10.1007/BF00303143.
5
The mechanism of cytoplasmic streaming in characean algal cells: sliding of endoplasmic reticulum along actin filaments.轮藻细胞中胞质环流的机制：内质网沿肌动蛋白丝滑动。
J Cell Biol. 1988 May;106(5):1545-52. doi: 10.1083/jcb.106.5.1545.
6
Substructure of cisternal organelles of neuronal perikarya in immature rat brains revealed by quick-freeze and deep-etch techniques.快速冷冻和深度蚀刻技术揭示未成熟大鼠脑神经元胞体池状细胞器的亚结构
Cell Tissue Res. 1989 Apr;256(1):53-64. doi: 10.1007/BF00224718.

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Adhesion between liposomes mediated by the chlorophyll a/b light-harvesting complex isolated from chloroplast membranes.由从叶绿体膜分离的叶绿素a/b捕光复合物介导的脂质体之间的粘附作用。
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Visualization of ribosomes by freeze-etching.通过冷冻蚀刻对核糖体进行可视化观察。
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A comparative study of freeze-etch replicas and thin sections of rat liver.大鼠肝脏冷冻蚀刻复制品与薄切片的比较研究。
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Structure of rough, smooth, stripped and reconstituted rough membranes derived from rat liver as visualized by the freeze fracture technique.通过冷冻蚀刻技术观察到的源自大鼠肝脏的粗糙、光滑、剥离和重构粗糙膜的结构。
Mol Biol Rep. 1975 Dec;2(4):311-9. doi: 10.1007/BF00357018.
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Freeze-etching nomenclature.冷冻蚀刻命名法。
Science. 1975 Oct 3;190(4209):54-6. doi: 10.1126/science.1166299.
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Intracellular aspects of the process of protein synthesis.蛋白质合成过程的细胞内层面
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8
Reversible particle movements associated with unstacking and restacking of chloroplast membranes in vitro.体外与叶绿体膜解堆叠和重新堆叠相关的可逆颗粒运动。
J Cell Biol. 1976 Oct;71(1):136-58. doi: 10.1083/jcb.71.1.136.
9
Spatial relationship of photosystem I, photosystem II, and the light-harvesting complex in chloroplast membranes.叶绿体膜中光系统I、光系统II和捕光复合体的空间关系。
J Cell Biol. 1977 May;73(2):400-18. doi: 10.1083/jcb.73.2.400.
10
Mobility of ribosomes bound to microsomal membranes. A freeze-etch and thin-section electron microscope study of the structure and fluidity of the rough endoplasmic reticulum.结合于微粒体膜的核糖体的移动性。粗面内质网结构与流动性的冷冻蚀刻及超薄切片电子显微镜研究。
J Cell Biol. 1977 Mar;72(3):530-51. doi: 10.1083/jcb.72.3.530.

核糖体结合位点在糙面内质网冷冻断裂膜上的可视化。

Ribosome binding sites visualized on freeze-fractured membranes of the rough endoplasmic reticulum.

作者信息

Giddings T H, Staehelin L A

出版信息

J Cell Biol. 1980 Apr;85(1):147-52. doi: 10.1083/jcb.85.1.147.

DOI:10.1083/jcb.85.1.147
PMID:7364870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110593/
Abstract

Freeze-fracture micrographs of cells of the green alga Micrasterias denticulata stabilized by ultrarapid freezing reveal imprints of polysomes on the rough endoplasmic reticulum membranes. The imprints appear as broad, spiral ridges on the P faces and as corresponding wide grooves on the E faces of the membranes. Distinct 110-A particles with a spacing of 270 +/- 45 A are associated with the P-face ridges. Where imprints of individual ribosomes can be discerned, it is seen that there is a 1:1 relationship between the ribosomes and the 110-A particles, and that the 110-A particles are located in a peripheral position with respect to the polysome spirals. We propose that the 110-A particles could be structural equivalents of ribosome-binding sites, consisting of a molecule each of ribophorins I and II and a nascent polypeptide chain. These observations suggest that the spiral form of polysomes could result from the forces generated by the extrusion of the growing polypeptide chains to one side of the polysome.

摘要

通过超快速冷冻稳定的绿藻齿牙微星藻细胞的冷冻蚀刻显微照片显示，多核糖体在糙面内质网膜上留下印记。这些印记在膜的P面上表现为宽阔的螺旋状脊，在E面上表现为相应的宽槽。间距为270±45 Å的明显的110 Å颗粒与P面脊相关。在可以辨别单个核糖体印记的地方，可以看到核糖体与110 Å颗粒之间存在1:1的关系，并且110 Å颗粒相对于多核糖体螺旋位于外围位置。我们提出，110 Å颗粒可能是核糖体结合位点的结构等效物，由核糖体结合蛋白I和II各一个分子以及一条新生多肽链组成。这些观察结果表明，多核糖体的螺旋形式可能是由生长的多肽链向多核糖体一侧挤出所产生的力导致的。