Kim C, Van Loon G R
Clin Chem. 1981 Jul;27(7):1284-7.
We describe a gas-chromatographic method, with electron capture detection, for determining 3-methoxy-4-hydroxyphenylethylene glycol in human urine. High sensitivity (5 pg per injection), recovery (93-97%), and reproducibility (CV 4.3%) are obtained. By this method, the 24-h urinary excretion of the free compound, the sulfate conjugate, the glucuronide conjugate, and the total in six normal male subjects average 0.15 (SEM 0.015), 1.09 (SEM 0.10), 1.22 (SEM 0.10), and 2.34 (SEM 0.21) mg, respectively. In three young women with amenorrhea/hirsutism, administration of dexamethasone decreased the total excretion of the compound.
我们描述了一种采用电子捕获检测的气相色谱法，用于测定人尿中的3-甲氧基-4-羟基苯乙二醇。该方法具有高灵敏度（每次进样5皮克）、高回收率（93 - 97%）和高重现性（变异系数4.3%）。通过此方法，6名正常男性受试者中游离化合物、硫酸酯共轭物、葡糖醛酸酯共轭物的24小时尿排泄量以及总量平均分别为0.15（标准误0.015）、1.09（标准误0.10）、1.22（标准误0.10）和2.34（标准误0.21）毫克。在3名患有闭经/多毛症的年轻女性中，给予地塞米松后该化合物的总排泄量降低。
