Vaughn D W, Green S, Kalayanarooj S, Innis B L, Nimmannitya S, Suntayakorn S, Rothman A L, Ennis F A, Nisalak A
Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok Children's Hospital, Thailand.
J Infect Dis. 1997 Aug;176(2):322-30. doi: 10.1086/514048.
A multicenter effort was begun in 1994 to characterize the pathophysiology of dengue using a study design that minimized patient selection bias by offering enrollment to all children with undifferentiated fever for <72 h. In the first year, 189 children were enrolled (age range, 8 months to 14 years). Thirty-two percent of these children had dengue infections (60 volunteers). The percentage of children with a secondary dengue infection was 93%, with only 4 (7%) having a primary dengue infection. The virus isolation rate from the plasma of children with dengue was 98%. Viremia correlated highly with temperature. All four dengue virus serotypes were isolated at both study sites. This study demonstrates that all four serotypes of dengue virus can cause dengue hemorrhagic fever, that all dengue patients as defined by serology experience viremia during the febrile phase, and that as fever subsides, so does viremia.
1994年启动了一项多中心研究，旨在通过采用一种研究设计来表征登革热的病理生理学，该设计通过让所有发热不超过72小时的未分化儿童入组，将患者选择偏倚降至最低。第一年，招募了189名儿童（年龄范围为8个月至14岁）。这些儿童中有32%患有登革热感染（60名志愿者）。二次登革热感染儿童的比例为93%，只有4名（7%）患有原发性登革热感染。登革热患儿血浆病毒分离率为98%。病毒血症与体温高度相关。在两个研究地点均分离出了所有四种登革热病毒血清型。这项研究表明，所有四种血清型的登革热病毒均可引起登革出血热，所有血清学定义的登革热患者在发热期均经历病毒血症，并且随着发热消退，病毒血症也随之消退。
