通过氨基化合物使纤连蛋白与明胶 - 琼脂糖解离。
Dissociation of fibronectin from gelatin-agarose by amino compounds.
作者信息
Vuento M, Vaheri A
出版信息
Biochem J. 1978 Oct 1;175(1):333-6. doi: 10.1042/bj1750333.
Abstract
Soluble fibronectin of human plasma was specifically dissociated at neutral pH from gelatin-agarose by several cationic amino compounds, notably the polyamines spermine, spermidine and putrescine, the basic amino acid arginine, and amino sugars. The neutral and acidic amino acids and the N-acetylated derivatives of amino sugars tested were ineffective. Gel-filtration experiments demonstrated that [14C]spermidine bound to fibronectin but not to gelatin.
摘要
人血浆中的可溶性纤连蛋白在中性pH条件下可被几种阳离子氨基化合物从明胶琼脂糖上特异性解离，特别是多胺类的精胺、亚精胺和腐胺、碱性氨基酸精氨酸以及氨基糖。所测试的中性和酸性氨基酸以及氨基糖的N - 乙酰化衍生物均无效。凝胶过滤实验表明，[14C]亚精胺与纤连蛋白结合，但不与明胶结合。
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