Hiersche H D, Nagl W
Arch Gynecol. 1980;229(2):83-90. doi: 10.1007/BF02109947.
Biopsies of hum endocervix were obtained from healthy women at day 5, 15, and 25 of the menstrual cycle, and immediately incubated with 3H-thymidine in a culture medium for 1 to 2 h. A few labelled nuclei were found in thick, semi-thin, and ultra-thin sections and indicated that gland cells of the columnar epithelium of the endocervix were capable of DNA synthesis and hence mitosis, independent of the menstrual cycle. As basal or "reserve" cells were less frequently labelled than differentiated epithelial cells, the findings suggested renewal of the secretory epithelium of the endocervix without cell replacement by basal or "reserve" cells. We discuss the implications of the results for the understanding of normal and neoplastic regeneration and for the treatment of cancer.
在月经周期的第5天、15天和25天从健康女性获取人子宫颈内膜活检组织，并立即在培养基中与3H-胸腺嘧啶核苷一起孵育1至2小时。在厚切片、半薄切片和超薄切片中发现了一些标记的细胞核，这表明子宫颈柱状上皮的腺细胞能够进行DNA合成，因此能够进行有丝分裂，且与月经周期无关。由于基底或“储备”细胞被标记的频率低于分化的上皮细胞，这些发现提示子宫颈分泌上皮的更新并非通过基底或“储备”细胞进行细胞替代。我们讨论了这些结果对于理解正常和肿瘤再生以及癌症治疗的意义。
