Turyk M E, Golub T R, Wood N B, Hawkins J L, Wilbanks G D
Rush Medical College, Department of Obstetrics and Gynecology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
In Vitro Cell Dev Biol. 1989 Jun;25(6):544-56. doi: 10.1007/BF02623567.
The human uterine cervix consists of an endocervical canal lined with a single layer of columnar mucus-secreting cells and an outer ectocervix covered by a stratified squamous epithelium. We report here the culture of human endocervical epithelial cells (HEnE) and human ectocervical epithelial cells (HEcE) in serum-free medium (KGM). Both HEnE and HEcE cultures were composed of keratinocytelike cells which formed desmosomal contacts and stratified in the presence of high concentrations of calcium ions. Cells with a pleomorphic epithelial morphology were observed in HEnE cultures, but not in HEcE cultures. Keratin 18, which is characteristic of endocervix in vivo, was detected by indirect immunofluorescent staining in all HEnE cells but was never detected in cultured HEcE. HEcE expressed keratin 13 which is characteristic of ectocervix in vivo. Although keratin 13 was never detected in primary HEnE cultures, it was expressed in passaged HEnE cultures grown in medium with high concentrations of calcium and in late passage HEnE cultures. HEnE underwent an average of 15.1 population doublings during serial culture. Mean colony-forming efficiency during Passages 2 to 3 was 14.7% and mean population doubling time was 17.8 h. HEcE cultures underwent significantly more population doublings (29.0) than HEnE cultures, whereas colony-forming efficiencies and doubling times were similar to those determined for HEnE. HEnE and HEcE cells may be useful in developing in vitro models of cervical squamous metaplasia and for exploring the interactions between target cell differentiation, carcinogens, and papillomaviruses in the development of cervical neoplasia.
人的子宫颈由内衬单层柱状黏液分泌细胞的子宫颈管和被复层鳞状上皮覆盖的外子宫颈组成。我们在此报告在无血清培养基（角质形成细胞生长培养基）中培养人子宫颈管上皮细胞（HEnE）和人子宫颈外上皮细胞（HEcE）的情况。HEnE和HEcE培养物均由形成桥粒连接并在高浓度钙离子存在下分层的角质形成细胞样细胞组成。在HEnE培养物中观察到具有多形上皮形态的细胞，但在HEcE培养物中未观察到。通过间接免疫荧光染色在所有HEnE细胞中检测到体内子宫颈管特有的角蛋白18，但在培养的HEcE中从未检测到。HEcE表达体内子宫颈外特有的角蛋白13。虽然在原代HEnE培养物中从未检测到角蛋白13，但在高钙培养基中生长的传代HEnE培养物和晚期传代HEnE培养物中表达。HEnE在连续培养期间平均经历15.1次群体倍增。第2至3代的平均集落形成效率为14.7%，平均群体倍增时间为17.8小时。HEcE培养物的群体倍增次数（29.0）明显多于HEnE培养物，而集落形成效率和倍增时间与HEnE测定的相似。HEnE和HEcE细胞可能有助于建立宫颈鳞状化生的体外模型，并用于探索宫颈肿瘤发生过程中靶细胞分化、致癌物和乳头瘤病毒之间的相互作用。
