Neat C E, Thomassen M S, Osmundsen H
Biochem J. 1980 Jan 15;186(1):369-71. doi: 10.1042/bj1860369.
Liver peroxisomes were prepared by using a Percoll gradient in a vertical rotor. beta-Oxidation was measured in peroxisomes isolated from livers of rats fed on either high-(15% by wt.) or low- (5% by wt.) fat diets. The feeding of high-fat diets gave a 1.4-2.4-fold increase in total liver peroxisomal beta-oxidation, and a similar increase in specific activity. A 1.5-4.5-fold increase was seen in the specific activity of purified peroxisomal preparations. The reasons for these increases are discussed.
通过在垂直转子中使用Percoll梯度制备肝脏过氧化物酶体。在从喂食高(15%重量)或低(5%重量)脂肪饮食的大鼠肝脏中分离出的过氧化物酶体中测量β-氧化。喂食高脂肪饮食使肝脏总过氧化物酶体β-氧化增加了1.4 - 2.4倍,比活性也有类似增加。纯化的过氧化物酶体制剂的比活性增加了1.5 - 4.5倍。讨论了这些增加的原因。