使用垂直转子从大鼠肝脏中快速制备过氧化物酶体。
The rapid preparation of peroxisomes from rat liver by using a vertical rotor.
作者信息
Neat C E, Osmundsen H
出版信息
Biochem J. 1979 May 15;180(2):445-8. doi: 10.1042/bj1800445.
Abstract
A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.
摘要
本文描述了一种通过在垂直转子中使用蔗糖密度梯度离心法从大鼠肝脏快速制备过氧化物酶体的方法。该制备物几乎不含线粒体和微粒体污染，可用于研究分离的过氧化物酶体的脂肪酸代谢。
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Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue.组织分级分离研究。17. 大鼠肝脏组织中单胺氧化酶、天冬氨酸转氨酶、丙氨酸转氨酶、D-氨基酸氧化酶和过氧化氢酶的细胞内分布。
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The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.从注射曲拉通WR-1339的大鼠肝脏中大规模分离过氧化物酶体、线粒体和溶酶体。改进的分离程序、自动分析、各组分的生化和形态学特性。
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Isolation of rat liver peroxisomes.大鼠肝脏过氧化物酶体的分离
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Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study.大鼠肝实质中肝细胞与非肝细胞之间细胞器和膜的分布。一项体视学研究。
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