Chaudry I H, Baue A E
Biochim Biophys Acta. 1980 Mar 20;628(3):336-42. doi: 10.1016/0304-4165(80)90383-9.
Intact rat soleus muscles or hemidiaphragms were incubated at 37 degrees C for 1 h in 1.0 ml of Krebs-HCO3 buffer containing 10 mM glucose, 5 mM (8-14C)-labelled ATP and (alpha-32P)-labelled ATP together with 5 mM MgCl2 under an atmosphere of 95% O2-5% CO2. Samples of the incubation medium and tissue extract were subjected to electrophoretic separation and the radioactivity present as adenine nucleotides was counted. Extensive degradation of the added nucleotides was observed in the presence of both tissues. The concentrations of 14C-labelled and 32P-labelled ATP found in the muscle and diaphragm indicated that ATP was present within the muscle and diaphragm cells. By maintaining higher concentrations of ATP in the medium, ATP uptake in both tissues exhibited a saturation-type kinetics. These results provide further evidence for intracellular uptake of ATP and also suggest that the transport of ATP into the cell could be a carrier-mediated process.
将完整的大鼠比目鱼肌或半膈在含有10 mM葡萄糖、5 mM（8-14C）标记的ATP和（α-32P）标记的ATP以及5 mM MgCl2的1.0 ml Krebs-HCO3缓冲液中，于95% O2 - 5% CO2气氛下在37℃孵育1小时。将孵育培养基和组织提取物样品进行电泳分离，并对作为腺嘌呤核苷酸存在的放射性进行计数。在两种组织存在的情况下均观察到添加的核苷酸发生大量降解。在肌肉和膈中发现的14C标记和32P标记的ATP浓度表明ATP存在于肌肉和膈细胞内。通过在培养基中维持较高浓度的ATP，两种组织中的ATP摄取均表现出饱和型动力学。这些结果为ATP的细胞内摄取提供了进一步的证据，也表明ATP进入细胞的转运可能是一个载体介导的过程。
