Tieche J M, Leonora J, Steinman R R
Endocrinology. 1980 Jun;106(6):1994-2005. doi: 10.1210/endo-106-6-1994.
A parotid hormone (PH-A beta) has been isolated and purified from porcine parotid glands. The biological effect of the hormone is the stimulation of a dentinal fluid transport (DFT) in molar teeth of rats. The method for purifying the PH-A beta includes: extraction at pH 7 and 5, ultrafiltration through Amicon membranes, ion exchange chromatography on carboxymethyl cellulose, gel filtration on Sephadex G-50, and preparative electrophoresis in polyacrylamide gel. The PH-A beta is characterized as a glycoprotein having a molecular weight of 8100 and containing 2% hexose. The amino acid composition suggests a molecule rich in glycine (46%) and proline (27.7%). Alanine, glutamic acid, arginine, serine, aspartic acid, leucine, and lysine account for the remaining 26% of the residues. The isoelectric point is pH 7.50. The partial N-terminal amino acid sequence was determined to be: NH2-Ala-Pro-Pro-Gly-Ala-Arg-Pro-Pro-Pro-Gly-Pro-Pro-Pro-Pro-Pro-Pro-(Glu)-(Pro)-?-(Pro)-(Pro)-Arg-(Pro)-(Pro)-(Pro)-Gly-(Gly)-(Gly). A 5.5 X 10(7)-fold increase in DFT activity has been achieved relative to the crude gland extract. As little as 9.7 pg PH-A beta are effective in stimulating DFT. The significance of the PH-A beta appears to be the maintenance of a functional DFT within teeth to allow the avascular dental tissues to meet their high metabolic requirements.
已从猪腮腺中分离并纯化出一种腮腺激素（PH-Aβ）。该激素的生物学效应是刺激大鼠磨牙中的牙本质液运输（DFT）。纯化PH-Aβ的方法包括：在pH 7和pH 5下提取、通过Amicon膜超滤、在羧甲基纤维素上进行离子交换色谱、在Sephadex G-50上进行凝胶过滤以及在聚丙烯酰胺凝胶中进行制备电泳。PH-Aβ的特征为一种糖蛋白，分子量为8100，含2%己糖。氨基酸组成表明该分子富含甘氨酸（46%）和脯氨酸（27.7%）。丙氨酸、谷氨酸、精氨酸、丝氨酸、天冬氨酸、亮氨酸和赖氨酸占其余26%的残基。等电点为pH 7.50。部分N端氨基酸序列确定为：NH2-Ala-Pro-Pro-Gly-Ala-Arg-Pro-Pro-Pro-Gly-Pro-Pro-Pro-Pro-Pro-Pro-(Glu)-(Pro)-?-(Pro)-(Pro)-Arg-(Pro)-(Pro)-(Pro)-Gly-(Gly)-(Gly)。相对于粗腺体提取物，DFT活性提高了5.5×10(7)倍。低至9.7 pg的PH-Aβ即可有效刺激DFT。PH-Aβ的意义似乎在于维持牙齿内功能性DFT，以使无血管的牙齿组织满足其高代谢需求。
