Lemieux R, Zarbl H, Millward S
J Virol. 1984 Jul;51(1):215-22. doi: 10.1128/JVI.51.1.215-222.1984.
Uncapped reovirus mRNA extracted at late times from infected L-cells is preferentially translated in extracts from infected L-cells. However, translation of this uncapped, late, reovirus mRNA in extracts from infected cells is sensitive to inhibition by the cap analog m7GTP . These results imply that reovirus infection does not induce a transition from cap-dependent to cap-independent translation. Nevertheless, the results of in vitro translational competition experiments between L-cell mRNA and late viral mRNA were consistent with the view that reovirus does induce an alteration in the cap-dependent translational apparatus of L-cells. The reduced efficiency of translation of a variety of capped mRNAs in extracts from infected cells is also consistent with this notion. We further conclude that a factor exists in reovirus-infected L-cells that specifically stimulates translation of uncapped reovirus mRNAs.
在感染后期从感染的L细胞中提取的无帽呼肠孤病毒mRNA在感染的L细胞提取物中优先被翻译。然而，这种无帽的、晚期的呼肠孤病毒mRNA在感染细胞提取物中的翻译对帽类似物m7GTP的抑制敏感。这些结果表明呼肠孤病毒感染不会诱导从帽依赖性翻译向帽非依赖性翻译的转变。尽管如此，L细胞mRNA与晚期病毒mRNA之间的体外翻译竞争实验结果与呼肠孤病毒确实诱导L细胞帽依赖性翻译装置改变的观点一致。感染细胞提取物中多种带帽mRNA翻译效率的降低也与此观点一致。我们进一步得出结论，在呼肠孤病毒感染的L细胞中存在一种因子，该因子特异性刺激无帽呼肠孤病毒mRNA的翻译。
