Bragt P C, Bonta I L, Adolfs M J
J Pharmacol Methods. 1980 Jan;3(1):51-61. doi: 10.1016/0160-5402(80)90064-9.
A granulomatous inflammation model is described. It is a modification of the cannulated sponge method and consists of a subdermally implanted teflon cylinder in the rat. This 'chamber' is cannulated and provided with holes to ensure contact and exchange of materials between the inner chamber and the surrounding tissues. The cannulae are exterioized at the back of the neck and allow materials to be injected and exudate to be collected at any given time during the development of granulomatous tissue around the chamber. Some parameters of this inflammation, such as exudation, granuloma growth, cellular infiltration in exudate, and the formation of prostaglandins were studied, and a comparison was made with the sponge implant model. The value of the chamber method is illustrated by brief reference to a study on the metabolism of 14C-arachidonic acid during granulomatous inflammation.
本文描述了一种肉芽肿性炎症模型。它是对插管海绵法的改良，由皮下植入大鼠体内的聚四氟乙烯圆柱体组成。这个“腔室”有插管并设有小孔，以确保内腔与周围组织之间的物质接触和交换。插管引出到颈部后方，使得在腔室周围肉芽肿组织形成过程中的任何给定时间都能注入物质并收集渗出液。研究了这种炎症的一些参数，如渗出、肉芽肿生长、渗出液中的细胞浸润以及前列腺素的形成，并与海绵植入模型进行了比较。通过简要提及一项关于肉芽肿性炎症期间14C-花生四烯酸代谢的研究，说明了腔室法的价值。
