Long W M, Bagby G J, Spitzer J J
Am J Physiol. 1980 May;238(5):H740-4. doi: 10.1152/ajpheart.1980.238.5.H740.
An approach is described that allows metabolic data obtained from a mixture of vital dye-excluding (T-) and nonexcluding (T+) myocytes to be extrapolated to a homogeneous cell population. Myocytes from adult dog hearts were dispersed by enzymatic treatment and separated into two fractions: one containing predominantly T-, and the other containing predominately T+ cells. Measuring the oxidation rate and viability of each fraction allows the determination of the rate of oxidation of a homogeneous cell population when palmitate, glucose, or lactate is the oxidizable substrate. The calculated rate of oxidation of these substrates by 100% T- cells was: 0.15, 0.46, and 2.99 nmol . mg protein-1 . min-1, respectively. Oxidation of palmitate and lactate by T+ cells was one-fifth of the T-cell rate. Glucose oxidation of T+ cells was not significantly different from zero. Use of this procedure will permit study of myocardial metabolism when experimental procedures may cause altered cell viability.
本文描述了一种方法，该方法可将从活力染料排斥（T-）和非排斥（T+）心肌细胞混合物中获得的代谢数据外推至同质细胞群体。成年犬心脏的心肌细胞经酶处理后分散，并分为两部分：一部分主要包含T-细胞，另一部分主要包含T+细胞。测量每一部分的氧化速率和活力，可确定以棕榈酸、葡萄糖或乳酸作为可氧化底物时同质细胞群体的氧化速率。计算得出，100% T-细胞对这些底物的氧化速率分别为：0.15、0.46和2.99 nmol·mg蛋白-1·min-1。T+细胞对棕榈酸和乳酸的氧化速率是T-细胞的五分之一。T+细胞对葡萄糖的氧化与零无显著差异。当实验操作可能导致细胞活力改变时，使用该程序将有助于研究心肌代谢。
