Esparza I, Brock J H
Biochim Biophys Acta. 1980 Apr 25;622(2):297-307. doi: 10.1016/0005-2795(80)90040-9.
The effect of trypsin digestion on iron-saturated and iron-free (apo) human, rabbit, bovine, pig and horse tranferrins has been studied. Iron-binding fragments were produced only from iron-saturated pig and bovine transferrins although some cleavage of the polypeptide chain occurred in all cases. The apo-transferrins were generally degraded to a greater extent than the corresponding iron-saturated proteins. The ability of the different transferrins to donate iron to rabbit reticulocytes varied in the order rabbit approximately pig greater than human approximately horse greater than bovine. Trypsin digestion considerably reduced the ability of pig and bovine transferrins to donate iron to rabbit reticulocytes, slightly reduced the iron-donating ability of rabbit transferrin, and had almost no effect on that of human or horse transferrins.
研究了胰蛋白酶消化对铁饱和及脱铁（脱辅基）的人、兔、牛、猪和马转铁蛋白的影响。仅从铁饱和的猪和牛转铁蛋白中产生了铁结合片段，不过在所有情况下多肽链均有一些裂解。脱铁转铁蛋白通常比相应的铁饱和蛋白降解程度更大。不同转铁蛋白向兔网织红细胞供铁的能力顺序为：兔≈猪＞人≈马＞牛。胰蛋白酶消化显著降低了猪和牛转铁蛋白向兔网织红细胞供铁的能力，轻微降低了兔转铁蛋白的供铁能力，而对人或马转铁蛋白的供铁能力几乎没有影响。
