Mineo J R, Camargo M E, Ferreira A W
Infect Immun. 1980 Feb;27(2):283-7. doi: 10.1128/iai.27.2.283-287.1980.
A polysaccharide fraction from Toxoplasma gondii was adsorbed to polystyrene plates, and the enzyme-linked immunosorbent assay was performed (poly-ELISA) with peroxidase-labeled anti-immunoglobulin G and anti-immunoglobulin M antibodies. A comparison was made with a T. gondii total protein extract ELISA (protein ELISA) in serum samples presenting different toxoplasmosis serological patterns, as indicated by a battery of tests for toxoplasmosis. Very low titers and negative results were seen for immunoglobulin G poly-ELISA both for serum samples corresponding to ancient or transitional-period infections (serological patterns II and III) and for samples of recent or acute toxoplasmosis (pattern I). On the contrary, immunoglobulin M poly-ELISA furnished high titer results for pattern I sera, and a very close agreement of titers was seen between immunoglobulin M protein ELISA and immunoglobulin M poly-ELISA. When the polysaccharide fraction was added to pattern I sera, a complete blocking of immunoglobulin M antibody reactivity resulted only for poly-ELISA. In the same way, the total protein extract could completely block only reactivity for protein ELISA. In both cases, a limited decrease in titers was observed for respective heterologous assays.
将来自刚地弓形虫的多糖组分吸附到聚苯乙烯板上，并用过氧化物酶标记的抗免疫球蛋白G和抗免疫球蛋白M抗体进行酶联免疫吸附测定（多聚酶联免疫吸附测定）。对呈现不同弓形虫病血清学模式的血清样本进行了比较，这些血清学模式通过一系列弓形虫病检测来表明。对于对应于既往或过渡时期感染（血清学模式II和III）的血清样本以及近期或急性弓形虫病样本（模式I），免疫球蛋白G多聚酶联免疫吸附测定均显示出非常低的滴度和阴性结果。相反，免疫球蛋白M多聚酶联免疫吸附测定为模式I血清提供了高滴度结果，并且免疫球蛋白M蛋白酶联免疫吸附测定和免疫球蛋白M多聚酶联免疫吸附测定之间的滴度非常接近。当将多糖组分添加到模式I血清中时，仅多聚酶联免疫吸附测定出现免疫球蛋白M抗体反应性完全阻断。同样，总蛋白提取物仅能完全阻断蛋白酶联免疫吸附测定的反应性。在这两种情况下，各自的异源测定中观察到滴度有限下降。
