Ybañez Rochelle Haidee, Nishikawa Yoshifumi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Institute of Molecular Parasitology and Protozoan Diseases, Main Campus and College of Veterinary Medicine, Barili Campus, Cebu Technological University, Cebu City 6000, Philippines.
Pathogens. 2022 Feb 21;11(2):277. doi: 10.3390/pathogens11020277.
Highly specific and sensitive diagnostic methods are vital for the effective control and treatment of toxoplasmosis. Routine diagnosis is primarily serological because infections stimulate persistently high IgG antibody responses. The sensitivity and specificity of methods are crucial factors for the proper diagnosis of toxoplasmosis, primarily dependent on the antigens used in different assays. In the present study, we compared the serodiagnostic performances of three recombinant dense granule antigens, namely, the GRA6, GRA7, and GRA14, to detect IgG antibodies against in human sera from the Philippines. Moreover, we evaluated the IgG1, IgG2, IgG3, and IgG4 responses against the different recombinant antigens, which has not been performed previously. Our results revealed that the TgGRA7 has consistently displayed superior diagnostic capability, while TgGRA6 can be a satisfactory alternative antigen among the GRA proteins. Furthermore, IgG1 is the predominant subclass stimulated by the different recombinant antigens. This study's results provide options to researchers and manufacturers to choose recombinant antigens suitable for their purpose.
高度特异且灵敏的诊断方法对于弓形虫病的有效控制和治疗至关重要。常规诊断主要是血清学诊断，因为感染会刺激产生持续高水平的IgG抗体反应。方法的敏感性和特异性是正确诊断弓形虫病的关键因素，主要取决于不同检测中使用的抗原。在本研究中，我们比较了三种重组致密颗粒抗原，即GRA6、GRA7和GRA14，用于检测菲律宾人血清中抗弓形虫IgG抗体的血清学诊断性能。此外，我们评估了针对不同重组抗原的IgG1、IgG2、IgG3和IgG4反应，这是之前未曾进行过的。我们的结果显示，TgGRA7始终表现出卓越的诊断能力，而TgGRA6在GRA蛋白中可以是一种令人满意的替代抗原。此外，IgG1是受不同重组抗原刺激产生的主要亚类。本研究结果为研究人员和制造商选择适合其目的的重组抗原提供了选择。
