Pierce N F, Cray W C, Engel P F
Infect Immun. 1980 Feb;27(2):632-7. doi: 10.1128/iai.27.2.632-637.1980.
Colera toxin was evaluated as an oral immunogen against experimental canine cholera. Dogs were immunized orally with 100-microgram doses of purified cholera toxin or comparable doses of crude toxin. Both doses caused moderate diarrhea in most nonimmune dogs. Repeated oral doses (12 doses in 54 days) gave marked protection against the diarrheal effect of oral toxin, provoked a vigorous antitoxic response in jejunal mucosa, and gave nearly complete protection against subsequent oral challenge with living virulent Vibrio cholerae. Protection appeared to be due largely to the antitoxic response in intestinal mucosa. The effectiveness of cholera toxin as an oral vaccine contrasts with the previously described ineffectiveness of toxoid given orally. This study provides an example of mucosal immunity due to a nonreplicating vaccine given orally and suggests that cholera toxin may be useful as a component of an oral vaccine for cholera.
霍乱毒素作为一种口服免疫原用于实验性犬霍乱的研究。用100微克纯化霍乱毒素或相当剂量的粗毒素对犬进行口服免疫。两种剂量在大多数未免疫的犬中均引起中度腹泻。重复口服剂量（54天内12剂）对口服毒素的腹泻效应具有显著保护作用，在空肠黏膜引发强烈的抗毒素反应，并对随后口服活的有毒霍乱弧菌的攻击提供近乎完全的保护。保护作用似乎主要归因于肠道黏膜中的抗毒素反应。霍乱毒素作为口服疫苗的有效性与先前描述的口服类毒素无效形成对比。本研究提供了一个因口服非复制疫苗而产生黏膜免疫的例子，并表明霍乱毒素可能作为霍乱口服疫苗的一个成分有用。
