Zelt D T, Owen J A, Marks G S
J Chromatogr. 1980 Feb 29;189(2):209-16. doi: 10.1016/s0021-9673(00)81520-4.
A high-performance liquid chromatographic system is described which is suitable for the separation and quantitative determination of a mixture of porphyrin esters in nanogram quantities from the culture medium used for maintenance of a monolayer culture of chick embryo liver cells. The desired sensitivity was obtained by coupling a high-performance liquid chromatograph with a second derivative-fluorometric detection system. The most effective method for preparation of the porphyrin methyl esters prior to chromatography was found to be lyophilization of the culture medium prior to esterification with 5% sulphuric acid in methanol.
本文描述了一种高效液相色谱系统，该系统适用于从用于维持鸡胚肝细胞单层培养的培养基中分离和定量测定纳克级卟啉酯混合物。通过将高效液相色谱仪与二阶导数荧光检测系统联用，可获得所需的灵敏度。发现在色谱分析之前制备卟啉甲酯的最有效方法是在用5%硫酸甲醇溶液进行酯化之前，先将培养基冻干。
