Gabriel E, Machlin L J, Filipski R, Nelson J
J Nutr. 1980 Jul;110(7):1372-9. doi: 10.1093/jn/110.7.1372.
Weanling rats were fed either a vitamin E-deficient diet or the deficient diet supplemented with vitamin E or the antioxidant ethoxyquin. Ethoxyquin was not effective in preventing the elevation in platelet number or percent aggregation. However, ethoxyquin was as effective as vitamin E in maintaining body weight, testes weight, pigmentation of the incisors and in preventing myopathy, observed either histologically or by an elevation in plasma glutamic oxalacetic transaminase (GOT) activity. Removal of ethoxyquin from the diet after 19 weeks of feeding resulted in a rapid onset of myopathy. These observations permitted us to study the requirement for vitamin E at various ages without the complication of any cumulative pathological effects of vitamin E deficiency. Ethoxyquin was deleted from the diet at 8, 20, 44 and 64 weeks of age. The requirement for vitamin E to reduce plasma GOT and pyruvate kinase (PK) activity was measured at 12, 24, 48 and 68 weeks of age. The requirement for vitamin E did not change significantly during this time period.
将断乳大鼠分为三组，分别喂食维生素E缺乏饮食、添加维生素E的缺乏饮食或添加抗氧化剂乙氧喹的缺乏饮食。乙氧喹在预防血小板数量增加或聚集百分比升高方面无效。然而，在维持体重、睾丸重量、门齿色素沉着以及预防组织学观察到的肌病或血浆谷草转氨酶（GOT）活性升高方面，乙氧喹与维生素E效果相同。喂食19周后从饮食中去除乙氧喹会导致肌病迅速发作。这些观察结果使我们能够研究不同年龄对维生素E的需求，而不会受到维生素E缺乏任何累积病理效应的干扰。在8、20、44和64周龄时从饮食中去除乙氧喹。在12、24、48和68周龄时测量降低血浆GOT和丙酮酸激酶（PK）活性所需的维生素E量。在此时间段内，对维生素E的需求没有显著变化。
