Machlin L J, Gabriel E, Spiegel H E, Horn L R, Brin M, Nelson J
J Nutr. 1978 Dec;108(12):1963-8. doi: 10.1093/jn/108.12.1963.
Weanling rats were fed vitamin E deficient diets for 6 to 15 weeks and then given vitamin E orally for 4 days. Plasma obtained 1 day after the last dose was assayed for glutamic oxalacetic transaminase (GOT) and pyruvate kinase activity (PK). Administration of vitamin E resulted in reduction in activity of both enzymes. Plasma levels of alkaline phosphatase, lactic dehydrogenase, and bilirubin were unaffected by vitamin E and there was no histological evidence of liver degeneration. The number of phagocytized muscle fibers was greatly reduced by vitamin E treatment, but a substantial number of necrotic fibers were still present. With more prolonged (8 days) treatment, plasma PK and GOT levels were reduced to levels found in plasma of vitamin E replete animals and few degenerated muscle fibers could be observed. It was concluded that resolution of the necrotizing myopathy in vitamin E deficient rats is a rapid process and that the decreased activity of PK and GOT in plasma is a sensitive indicator of the resolution process. The decrease in plasma enzyme levels is an easily quantitated and reproducible biological response to vitamin E administration. Thus, this approach provides a basis for a sensitive and accurate bioassay for vitamin E activity.
将断乳大鼠用缺乏维生素E的饲料喂养6至15周，然后口服维生素E 4天。在最后一剂维生素E给药1天后采集的血浆，用于检测谷氨酸草酰乙酸转氨酶（GOT）和丙酮酸激酶活性（PK）。给予维生素E导致这两种酶的活性降低。血浆碱性磷酸酶、乳酸脱氢酶和胆红素水平不受维生素E的影响，并且没有肝脏变性的组织学证据。维生素E治疗使吞噬的肌纤维数量大大减少，但仍存在大量坏死纤维。经过更长时间（8天）的治疗，血浆PK和GOT水平降至维生素E充足动物血浆中的水平，并且几乎观察不到变性的肌纤维。得出的结论是，维生素E缺乏大鼠坏死性肌病的消退是一个快速过程，血浆中PK和GOT活性的降低是消退过程的敏感指标。血浆酶水平的降低是对维生素E给药的一种易于定量且可重复的生物学反应。因此，这种方法为维生素E活性的灵敏且准确的生物测定提供了基础。
