Boeynaems J M, Oates J A, Hubbard W C
Prostaglandins. 1980 Jan;19(1):87-97. doi: 10.1016/0090-6980(80)90156-2.
5-, 8-, 9-, 11-, 12- AND 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2 and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (mu Porasil) column or on a reversed phase (mu Bondapak C18) column. The yield of 5-HPETE was considerably greater when the mu Bondapak C18 column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20 degrees C, the HPETES were stable for several months.
5-、8-、9-、11-、12-和15-氢过氧化二十碳四烯酸（HPETEs）通过花生四烯酸与过氧化氢和铜离子反应生成。它们通过高效液相色谱法在硅胶（μPorasil）柱或反相（μBondapak C18）柱上进行纯化。使用μBondapak C18柱时，5-HPETE的产率要高得多。通过氧化三苯基膦的能力对HPETEs进行表征和测定：通过气相色谱法监测三苯基膦氧化物的形成。当储存在-20℃的二氯甲烷中时，HPETEs可稳定保存数月。
