关于局部应用药物对皮脂腺细胞动力学的影响。以叙利亚仓鼠耳部二硫化硒为例的研究。

On the influence of topically applied drugs on cell kinetics in the sebaceous gland. Investigations with the example of selenium disulfide in the Syrian hamster ear.

作者信息

Gloor M, Gantner M, Wirth H, Schnyder U W

出版信息

Dermatologica. 1980;160(3):175-9. doi: 10.1159/000250492.

Abstract

60 male Syrian hamsters were treated on one ear with 2.5% selenium disulfide in a surfactant base and on the other ear with the same surfactant base. After treatment 15 animals were investigated by 3H-thymidine autoradiography, 15 animals by the colcemid method and 15 animals by the in vivo double labelling technique with 3H- and 14C-thymidine. In 15 animals the porportion of labeled cells was determined which still had contact with the basal lamina in the sebaceous gland 6 days after 3H-thymidine injection. Besides an increase in sebaceous gland size and in cell proliferation, a shortening of the S phase and a reduction of the labeled sebaceous gland cells which still have contact with the basal lamina of the sebaceous gland 6 days after 3H - thymidine injection were determined.

摘要

60只雄性叙利亚仓鼠，一只耳朵用含2.5%二硫化硒的表面活性剂基质处理，另一只耳朵用相同的表面活性剂基质处理。处理后，15只动物通过3H-胸腺嘧啶核苷放射自显影术进行研究，15只动物通过秋水仙酰胺法进行研究，15只动物通过3H-和14C-胸腺嘧啶核苷的体内双标记技术进行研究。在15只动物中，测定了在注射3H-胸腺嘧啶核苷6天后仍与皮脂腺基底层接触的标记细胞的比例。除了皮脂腺大小和细胞增殖增加外，还测定了S期缩短以及在注射3H-胸腺嘧啶核苷6天后仍与皮脂腺基底层接触的标记皮脂腺细胞减少。