Jacobs F A, Flynn T G, Clark A F
Mol Cell Endocrinol. 1980 Jun;18(3):177-87. doi: 10.1016/0303-7207(80)90064-7.
We have investigated the effects of castration and androgen replacement on the kinetic characteristics of rat prostatic acid phosphatases (AP). Chromatography on DEAE-cellulose DE-23 and on Sephacryl S-200 allowed the separation of lysosomal (S-1) and secretory (S-3) forms of AP. In addition, these techniques revealed a third enzymic form (S-2), which eluted in the void volume of the Sephacryl S-200 column and which occurred in significant amounts only 15 days after castration. The S-2 form was extremely resistant (Ki 1500 microM) to L-tartrate inhibition in the 15-day post-castration rat which is in contrast to the behaviour of S-2 (Ki 145 microM) in control animals. The appearance of this unidentified AP form can explain the dramatic drop in the per cent inhibition of total rat AP observed between 7 and 15 days post-castration.
我们研究了去势及雄激素替代对大鼠前列腺酸性磷酸酶（AP）动力学特性的影响。通过在DEAE-纤维素DE-23和Sephacryl S-200上进行色谱分离，可将溶酶体形式（S-1）和分泌形式（S-3）的AP分离出来。此外，这些技术还揭示了第三种酶形式（S-2），它在Sephacryl S-200柱的空体积中洗脱出来，并且仅在去势后15天大量出现。在去势后15天的大鼠中，S-2形式对L-酒石酸抑制具有极强的抗性（Ki为1500微摩尔），这与对照动物中S-2（Ki为145微摩尔）的行为形成对比。这种未鉴定的AP形式的出现可以解释在去势后7至15天观察到的大鼠总AP抑制百分比的急剧下降。
