[Intensification of peroxidation and changes in lipid composition in ischemic liver homogenates and subcellular fractions].

Lipid peroxidation (hydroperoxides and antioxidant activity) and lipid composition in liver homogenates and in its mitochondrial, microsomal and nuclear fractions were studied after 15--120 minutes of liver warm ischemia. Pronounced enhancement of lipid peroxidation during ischemia was seen in all subcellular fractions, particularly in the mitochondrial one. The results are consistent with low resistance of the liver and specifically its mitochondria to ischemia. Activation of lipid peroxidation corresponded with changes in the lipid composition. Decrease in the level of total and readily oxidizable lipids (cardiolipin) and accumulation of poorly oxidizable phospholipids (sphingomyelin) and highly toxic lysophosphatides were recorded. The evidence obtained suggests a role for lipid peroxidation in the pathogenesis of ischemic injuries to the liver.