Meibach R C, Glick S D, Ross D A, Cox R D, Maayani S
Brain Res. 1980 Aug 11;195(1):167-76. doi: 10.1016/0006-8993(80)90874-4.
Experiments were conducted to determine the optimal conditions necessary for implementing modifications of the 2-deoxyglucose (2-DDG) technique. Substitution of tritium-labeled 2-DDG with subsequent microdissection of selected brain regions and liquid scintillation counting produced results that were highly correlated with both [14C]radioautograms and glucose utilization values as obtained by Sokoloff et al. The route of administration of isotope was also varied. Whole brain uptake at maximal levels of incorporation was the same for both intravenously and intraperitoneally injected animals. Radioautograms from i.p. and i.v. injected animals were indistinguishable. Densitometric analyses of the i.p. radioautograms were highly correlated with glucose utilization values. Thus, relative indices of functional activity may be obtained when experimental circumstances preclude arteriovenous cannulations and restraint. The use of naive, unrestrained animals, therefore, makes the 2-DDG technique applicable to a broader range of studies.
进行了实验以确定实施2-脱氧葡萄糖（2-DDG）技术改进所需的最佳条件。用氚标记的2-DDG替代，随后对选定的脑区进行显微解剖并进行液体闪烁计数，所得结果与Sokoloff等人获得的[14C]放射自显影片和葡萄糖利用值高度相关。同位素的给药途径也有所不同。静脉注射和腹腔注射的动物在最大掺入水平时全脑摄取量相同。腹腔注射和静脉注射动物的放射自显影片无法区分。腹腔注射放射自显影片的光密度分析与葡萄糖利用值高度相关。因此，当实验条件不允许进行动静脉插管和约束时，可以获得功能活动的相对指标。因此，使用未经处理、不受约束的动物使2-DDG技术适用于更广泛的研究。
