Chatterjee A, Maiti I B
Department of Biochemistry, Bose Institute, Calcutta, India.
Mol Cell Biochem. 1987 Nov;78(1):55-63. doi: 10.1007/BF00224424.
Catfish hepatic metallothionein was purified to homogeneity by Sephadex G-75 gel filtration, DEAE-Sephadex A-25 column chromatography and preparative polyacrylamide gel electrophoresis. Induction by cadmium and zinc, characteristic UV spectrum, cadmium binding property and its low MW established that it was a metallothionein. Antibody was raised in rabbit against catfish metallothionein. Catfish antimetallothionein cross-reacted with other fish metallothioneins but not with chicken or rodent metallothionein. Catfish metallothionein is more electronegative as compared to mouse, rat, chicken or hamster metallothionein. Catfish MT appeared to aggregate readily on storage and to be less electronegative.
通过葡聚糖凝胶G - 75凝胶过滤、二乙氨基乙基葡聚糖A - 25柱色谱和制备型聚丙烯酰胺凝胶电泳，将鲶鱼肝脏金属硫蛋白纯化至同质。镉和锌的诱导作用、特征紫外光谱、镉结合特性及其低分子量表明它是一种金属硫蛋白。用鲶鱼金属硫蛋白对兔进行免疫以产生抗体。鲶鱼抗金属硫蛋白与其他鱼类金属硫蛋白发生交叉反应，但与鸡或啮齿动物的金属硫蛋白不发生交叉反应。与小鼠、大鼠、鸡或仓鼠的金属硫蛋白相比，鲶鱼金属硫蛋白的电负性更强。鲶鱼金属硫蛋白在储存时似乎容易聚集，且电负性较小。
