Ito T, Eggena P, Barrett J D, Katz D, Metter J, Sambhi M P
Hypertension. 1980 Jul-Aug;2(4):432-6. doi: 10.1161/01.hyp.2.4.432.
The presence of a renin-angiotensin system in the central nervous system (CNS) has been demonstrated by several investigators, but little is known regarding the origin of its components. In this study we have compared the immunological and physical-chemical nature of angiotensinogen in plasma and cerebrospinal fluid (CSF) of human subjects and explored whether differences are present in CSF angiotensinogen concentrations of normal and hypertensive subjects. No significant differences in the nature of plasma and CSF angiotensinogen was observed with respect to molecular weight (65-70,000) electrophoretic mobility (RFalb = 0.67 plus or minus 0.003) or angiotensin I (AI) generated (pI = 6.6). Following isoelectric focusing, the plasma angiotensinogen was shown to consist of a single component with an isoionic point of 4.40 plus or minus 0.04. CSF angiotensinogen, on the other hand, resolved into three components (pI = 4.76 plus or minus 0.02; 5.16 plus or minus 0.04; 5.76 plus or minus 0.04). Although no correlations were observed between angiotensinogen levels in the CSF or plasma with blood pressure (BP), a statistically significant difference in angiotensinogen concentration of both plasma and CSF was observed between normotensive and hypertensive subjects. The differences in the chemical and immunological nature of human plasma and CSF angiotensinogens suggest that the angiotensinogen of CSF is not of peripheral origin.
几位研究者已证实中枢神经系统（CNS）中存在肾素-血管紧张素系统，但其组分的来源却鲜为人知。在本研究中，我们比较了人类受试者血浆和脑脊液（CSF）中血管紧张素原的免疫学和物理化学性质，并探究了正常人和高血压患者的脑脊液血管紧张素原浓度是否存在差异。在分子量（65 - 70,000）、电泳迁移率（RFalb = 0.67 ± 0.003）或生成的血管紧张素I（AI）（pI = 6.6）方面，未观察到血浆和脑脊液血管紧张素原性质的显著差异。等电聚焦后，血浆血管紧张素原显示为由单一成分组成，等离子点为4.40 ± 0.04。另一方面，脑脊液血管紧张素原分解为三个成分（pI = 4.76 ± 0.02；5.16 ± 0.04；5.76 ± 0.04）。虽然未观察到脑脊液或血浆中血管紧张素原水平与血压（BP）之间的相关性，但在正常血压和高血压受试者之间观察到血浆和脑脊液血管紧张素原浓度存在统计学上的显著差异。人类血浆和脑脊液血管紧张素原在化学和免疫学性质上的差异表明，脑脊液中的血管紧张素原并非来源于外周。
