Ray J H, Altenburg L C
Mutat Res. 1978 Dec;54(3):343-54. doi: 10.1016/0165-1161(78)90024-9.
Sodium selenite (Na2SeO3) sister-chromatid exchange (SCE) induction was studied in both short-term and long-term cell cultures. The ability of Na2SeO3 to induce SCEs was found to depend on the culture conditions employed. Concentrations of Na2SeO3 (7.90 X 10(-6) M and greater) that produced elevated SCE frequencies in whole blood cultures resulted in control level SCE frequencies (6-8 SCEs/cell) in Ficoll-Hypaque--purified lymphocyte cultures. However, whole blood and purified lymphocyte cultures were equally sensitive to SCE induction by methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS), and N-hydroxy-2-acetylaminofluorene (N-OH-AAF). Analysis of different whole blood components showed that the presence of red blood cells (RBCs), and specifically RBC lysate, was a prerequisite for Na2SeO3 SCE induction in purified lymphocyte cultures. The SCE frequencies of xeroderma pigmentosum (XP12RO) and normal human lymphoblastoid cell lines were also found to be unaffected by Na2SeO3 concentrations that produced elevated SCE frequencies in whole blood cultures. Incubation of these latter two cell types with Na2SeO3 and RBC lysate resulted in SCE frequencies comparable to those in Na2SeO3-exposed whole blood cultures.
在短期和长期细胞培养中研究了亚硒酸钠（Na2SeO3）对姐妹染色单体交换（SCE）的诱导作用。发现Na2SeO3诱导SCE的能力取决于所采用的培养条件。在全血培养中能使SCE频率升高的Na2SeO3浓度（7.90×10(-6) M及更高），在Ficoll-泛影葡胺纯化的淋巴细胞培养中却导致SCE频率处于对照水平（6 - 8个SCE/细胞）。然而，全血和纯化的淋巴细胞培养对甲磺酸甲酯（MMS）、甲磺酸乙酯（EMS）和N - 羟基 - 2 - 乙酰氨基芴（N - OH - AAF）诱导SCE的敏感性相同。对不同全血成分的分析表明，红细胞（RBC）的存在，特别是红细胞裂解物，是在纯化淋巴细胞培养中Na2SeO3诱导SCE的前提条件。还发现，在全血培养中能使SCE频率升高的Na2SeO3浓度对着色性干皮病（XP12RO）和正常人淋巴母细胞系的SCE频率没有影响。将后两种细胞类型与Na2SeO3和红细胞裂解物一起孵育，其SCE频率与暴露于Na2SeO3的全血培养中的频率相当。
