Crampton E L, Glass R C, Marchant B, Rees J A
J Chromatogr. 1980 Aug 8;183(2):141-48. doi: 10.1016/s0378-4347(00)81687-4.
A method is described for the measurement of the antidepressant drug dothiepin in human plasma. The procedure involves the addition of deuterodothiepin as an internal standard, extraction and measurement by chemical ionisation mass fragmentography. The minimum measurable concentration of 0.5 ng/ml enabled the pharmacokinetics of dothiepin in man to be studied after a single oral dose of 75 mg of dothiepin hydrochloride. Unlike other tricyclic antidepressants the apparent half-life of elimination (approximately 24 h) showed very little intersubject variation. However, the apparent volume of distribution was much more variable and could account for the wide range of steady state concentrations which have been found in patients taking dothiepin.
本文描述了一种测定人体血浆中抗抑郁药物多塞平的方法。该方法包括加入氘代多塞平作为内标，通过化学电离质谱碎片分析法进行提取和测定。最低可测浓度为0.5 ng/ml，这使得在单次口服75 mg盐酸多塞平后能够研究多塞平在人体内的药代动力学。与其他三环类抗抑郁药不同，消除的表观半衰期（约24小时）个体间差异很小。然而，表观分布容积的变化要大得多，这可以解释服用多塞平的患者中稳态浓度范围较宽的现象。
