血小板冷冻保存简化：一种改良的甘油-葡萄糖法。
Cryopreservation of platelets simplified: a modified glycerol-glucose method.
作者信息
Ridgway R L, Usry R T
出版信息
Transfusion. 1980 Jul-Aug;20(4):427-32. doi: 10.1046/j.1537-2995.1980.20480260274.x.
Platelets were prepared for freezing in liquid nitrogen by prediluting with 1 ml glycerol-glucose freezing solution, allowing the platelet concentrate to set for ten minutes at 29 C, and then adding an additional 8 ml glycerol-glucose freezing solution. A 3-mm thick cardboard holder was constructed and used to hold the platelet concentrate during immersion and storage in liquid nitrogen. The mean recovery of frozen thawed platelets was 79 per cent. Electron microsopic examination of the frozen thawed platelets showed that the platelets to be structurally intact. In vitro testing showed the platelets to be functional.
血小板通过以下步骤准备用于在液氮中冷冻
先用1毫升甘油 - 葡萄糖冷冻溶液进行预稀释，使血小板浓缩物在29℃下放置十分钟，然后再加入8毫升甘油 - 葡萄糖冷冻溶液。制作了一个3毫米厚的硬纸板支架，用于在将血小板浓缩物浸入液氮及储存期间固定它。冷冻解冻后的血小板平均回收率为79%。对冷冻解冻后的血小板进行电子显微镜检查显示血小板结构完整。体外测试表明血小板具有功能。
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